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Information for Authors

The journal publishes concise original research reports, authoritative reviews, technical developments and commissioned book reviews of the effects produced by low temperatures on a wide variety of biophysical and biological processes, or studies involving low temperature techniques in the investigation of biological and ecological topics. The Journal will accept papers that reach a high standard of scientific endeavour as judged by international peer review.

Contributions are peer-reviewed by an international editorial board and referees, and normally published within eight weeks of receiving an acceptable manuscript. There are no page charges and we offer a free pdf. file to authors. Circulation is world-wide and the journal is abstracted by all major services.

This page is also available in printable (Microsoft Word) format: Instructions for Authors (.doc)

Manuscript Layout

  1. Papers will be edited directly from authors' electronic submissions, using the downloadable template which therefore must conform to a standardised house-style, be accurate, clear and well laid out. For speed of publication, no proofs are printed, and final responsibility for accuracy rests with author(s) who may be contacted by the editor for clarification during sub-editing.
  2. Authors have access to a downloadable template for authors which must be used for submission of their article . Double column format is directed simply by the using template (see Template Instructions below - point 16 onwards) with page numbering .
  3. Contributions must be concise and no longer than 12 pages. Because of pressure on space a maximum of 12 pages including figures, tables and references is allowed. Manuscripts longer than 12 pages will be returned to the author for shortening unless prior agreement has been reached with the editor.
  4. Electronic submission is essential, using the downloadable template. Manuscripts should be singlespaced with the text in Times (New) Roman 12 point font. Manuscripts which do not conform to this specification may be returned for resetting. (In the event of difficulty in conforming to the specification, contact the editorial office for help). All Final Printing will normally be in Black and White . Images can only be accepted for Colour Printing by prior arrangement to pay the costs of printing (150 per page).  Images should only be submitted in colour if the authors state that they agree to pay for the colour images in the Letter of Submission. This decision must be taken by the authors before submitting the manuscript for assessment to ensure consistency in the refereeing procedure.
  5. Author Open Access. Authors may choose to select Open Access option at the time of manuscript submission. A modest fee is charged to cover administrative costs. You will be asked  to confirm that you will pay the open access fee (600 Euros ) when invoiced. You will need to choose the 'Open Access' option when you first submit your paper to the Editorial Office. The article will be made available by Ingenta Open Access on receipt of the fee.
  6. The title page should have:
    1. The TITLE (in 14-point font) in BOLD CAPITALS CENTRED on the page with a 50 mm top margin
    2. This should be followed by the Author(s)' names (regular font) separated from the title by a single line space, centred and indicating by an asterisk* the author to whom correspondence should be addressed. The last author name should be preceded by 'and'. To assist information retrieval, titles should be specific and informative
    3. The full correspondence address(es) should left-adjusted and separated by a single line space from the author names. Additionally, an email contact address must be given. Superscripted numeral should be used to indicate which addresses are associate with which authors
    4. An Abstract of no more than 150 words, with bold, initial capital centred heading. The abstract should be specific and informative to assist retrieval
    5. Up to six Keywords: (heading left adjusted) continuing the line.
  7. The text should normally be divided into conventional Introduction, Materials and Methods, Results, Discussion and References. These headings should be BOLD, CAPITAL and CENTRED. Any Acknowledgements: (heading left adjusted) should appear after the text (before the References) continuing the line.
  8. The text (see Template Instructions - point 16 onwards) should be fully justified and continuous with no spaces between paragraphs, the first line of which should be indented approximately four spaces. Subheadings should be in lower case italic with initial capital. Sub-subheadings should be in regular scipt underlined with initial capital and conclude with colon or full stop; text should then continue the line. If you use a font other than Times Roman or Arial to prepare the document, please ensure that all characters are properly converted to Times Roman or Arial before submitting the document to the editorial office. Please use the correct characters for degree Celsius (C) not the superscripted o. Statistical symbols should be italicised according to this example (P, F, t ,χ).
  9. Tables should be included in the text. All tables (titles, footnotes, table entries) should be clearly distinguished from the main text by changing to a sans-serif font such as Arial 11-point (this is an example to allow 10% reduction by the printer without loss of legibility, and separated by at least one line from the text above and below. Do not include vertical lines, and keep horizontal lines to the minimum to distinguish the column headings.
  10. Figures, graphs, structural formulae etc., should be prepared by computer using a common spreadsheet or graphics package such as Microsoft Excel (see Template Instructions - point 16 onwards) . Remove all horizontal scale lines from graphs and do not box in either the axes or the whole figure. Line drawings should be sufficiently bold to remain clear after 10% reduction. Figure legends (axis labels, keys) should be clearly distinguished from the main text by changing to a sans-serif font such as Arial 11-point allowing 10% reduction by the printer without loss of legibility. In general unless there is reason to choose 'standard deviation' for error bars standard error of the mean (abbreviated SEM) is preferred. Figure legends should carry enough information to be understood without detailed reference to the text. Numbers of replicates for calculation of the statistical significance should be given. Figures should be included directly in the text. IT IS VERY IMPORTANT THAT YOU CHECK THE APPEARANCE OF THE FONTS USED AFTER IMPORTING THE FIGURE INTO THE FILE TO ENSURE THEY ARE OF SUFFICIENT SIZE STILL TO BE LEGIBLE AFTER A 10% REDUCTION. If you use a font other than Times Roman or Arial to prepare the document, please ensure that all characters are properly converted to Times Roman or Arial before submitting the document to the editorial office.
  11. Photographs should be submitted electronically embedded in the text (see Template Instructions - point 16 onwards) Authors should bear in mind that the page size before reduction is 160 x 250mm and the photograph should fit within that area allowing space for legend. If at all possible, photographs should be grouped together, bearing in mind the maximum possible size. Good contrast is imperative. Labelling should be black or white according to maximum contrast in Arial 11-point font, or similar. For micrographs, a scale bar indicating magnification should be shown on the photographic print. If several images are used in a figure, they should be elec tronically grouped.
    Units: SI units are preferred. Consistency is essential.
  12. References should be referred to in the text by number in parenthesis (5, 21) and should be arranged after the text in alphabetical order and numbered 1...n in the form indicated by the following examples (all authors should be listed). Standard abbreviations for journal titles may be used:
    • Reed BM (2001) Cryoletters 22, 97-104.
    • Thurston LM, Watson PF & Holt WV (2003) Theriogenology 60, 101-113.
    • Songsasen N, Tong J & Leibo S (1998) J Exp Zool 280, 189-196
    • Withers LA & Engelmann F (1997) in Agricultural Biotechnology, (ed) A Altman, Marcel Dekker Inc, New York, pp 57-88.
  13. The Journal maintains its high standard by means of expert refereeing by an international panel. After review, if considered worthy of publication the manuscript will normally be returned for revision as recommended by the referee(s).
  14. Revised manuscripts must be marked up to show revisions as requested by the Editorial Office. CryoLetters is a rapid publication journal and the responsibility for the appearance of the final revised article rests with the Authors.
  15. Ethical standards
    1. All experimental work involving animals should conform to European Convention on the use of Experimental Animals and the Directive (PDF) that seeks to implement it. Where appropriate, authors should state that animal experiments were conducted in accordance with a named National Policy or Licensing Authority and were approved by the Institutional Ethics Committee. Experimental methods should include details of anaesthetic (generic name, dose, route of administration) and surgical procedures. Chilling alone is not an acceptable method for rendering vertebrates insentient; an anaesthetic agent must be used. Work on isolated tissues, including primary cell cultures, must state from where and how the materials were derived.
    2. oAll work involving humans or human tissues or cells must contain in the text a statement that approval for the study has been obtained from the appropriate National or Institutional Ethics Committee and conforms to the standards set by the Declaration of Helsinki (last modified in 2004), and that material was obtained only with Informed Consent in writing for each individual in the study.
    3. The editors reserve the right to refuse publication of any work which  does not conform to these essential ethical standards.
  16. How to use the new CryoLetters templates
  17. Download templates:

    Using the Main Text template

    1. The templates were designed to facilitate the authors of CryoLetters to prepare their camera-ready typescripts in a standardized format. The templates make it easy for one to layout the text, figures, tables, pictures and legends. They conform to the format of the journal, and the type and size of fonts required.
    2. The template is written in Microsoft Word 97-2003 Document and is compatible with most other versions. Copy the file to C: programs/ Microsoft office/templates. To use it, open Word first, go to File, click New, and browse the template. Alternatively, you can copy it in any file folder where you would like to store. To use it, you simply click the template file twice.
    3. Click on any macrobutton [words in the brackets] and start typing (e.g. title, bylines, abstract, introduction, subtitles and etc.). When a macrobutton is clicked, it will be highlighted. When you start to type, the macro will disappear automatically. Macrobuttons specify certain formats, therefore do not delete them before writing. The specification though is fixed (e.g. font type, font size, spacing, margin etc.). Treat the template as a regular file, subscript, superscript, special symbols and other variations could be done just like in any word document. As long as the template is not altered, the format will be maintained.
    4. Not every manuscript follows the same sequence: Summary, INTRODUCTION, MATERIALS AND METHODS, RESULTS, and DISCUSSION . The words in the heading can be changed as you wish. Macrobuttons can be copied and pasted to elsewhere.
    5. For references, the number will be listed automatically. The authors can add or delete freely any reference without bothering to change the numbering of the references. If cut/copy and paste functions are used to sort out the alphabetical order of the references, the number of each reference will change automatically. If you are using any reference software to prepare your list of references, convert the plain text file first, you can then use the copy and paste function.

    Using the Tables, Photos and Figures template

    1. Blue frames are prepared in a separate template for the layout of figures and legends. The simplest way is to use one frame for each display item. Should you need more frames, click the frame and point the cursor to the shaded border of the frame, use copy/paste function to have more. To save your time, you are advised to finish your text first, and then to layout the display item with frames. You can copy, paste and move the frames to where you wish to place in the paper.
    2. If the display item (including photo) is too big and you need to reduce its size, the frame will do it for you. Copy the over-sized display item and past onto the frame. You will see that your over-sized item is now fitted perfectly. To allow you write a legend, drag the bottom edge of the frame down to have more space (the size of the figure will not change). Note the edge of the frame is different from the edge of the display item. If you drag the edge of a display item, the shape of the display item will change.

Submission of Manuscripts

Electronic submission is essential to the Editorial Office or the appropriate Regional Associate Editor, together with a completed copy of the  Declaration and Copyright Transfer form (Microsoft Word document).

Executive Editor

  • Professor Barry Fuller, Royal Free & UCL Medical School, Department of Surgery, London NW3 2QG. Email:

Assistant Editors

  • Prof H.W. Pritchard, Royal Botanic Gardens Kew, Wakehurst Place, West Sussex, UK
  • Prof F Engelman, IRD, Montpelier, France
  • Dr Wendell Sun, Shanghai, China



A free .pdf file will be sent to the author marked by an asterisk*. Additional printed reprints can be purchased by completion of the order form enclosed with the manuscript acceptance notice. Requests for reprints cannot be accepted after publication of the article.

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Site updated: 22 November, 2020


Volume 41 (2020)
Volume 40 (2019)
Volume 39 (2018)
Volume 38 (2017)
Volume 37 (2016)
Volume 36 (2015)
Volume 35 (2014)
Volume 34 (2013)
Volume 33 (2012)
Volume 32 (2011)
Volume 31 (2010)
Volume 30 (2009)
Volume 29 (2008)
Volume 28 (2007)
Volume 27 (2006)
Volume 26 (2005)
Volume 25 (2004)
Volume 24 (2003)
Volume 23 (2002)
Volume 22 (2001)
Volume 21 (2000)
Volume 20 (1999)

For Abstracts published from meetings, such as SLTB meetings, go to the relevant Volume Year  of the journal (above).
Abstracts are often published by the journal in the Year subsequent to the Meeting's Date

For Full text Free Access Content (from 2000 onwards) go to CryoLetters at Ingenta and look for the blue symbol.